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Association of Physical Activity and Total Cholesterol with VO2Max in Middle-Aged Women: A Cross-Sectional Study

Authors

  • Moh. Eko Cahyo Pujianto Department of Sports Science, Faculty of Sports Science, Universitas Negeri Malang, Indonesia
  • Rias Gesang Kinanti Faculty of Medicine, Universitas Negeri Malang, Indonesia

DOI :

https://doi.org/10.62255/mjhp.v4iSuppl%201.325
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Abstract

Background: Declining cardiorespiratory fitness in women over 45 is linked to aging, hormonal changes, low physical activity, and elevated cholesterol, which collectively impair oxygen consumption (VO?Max). Physical activity may enhance cardiorespiratory efficiency, while high cholesterol can compromise oxygen distribution through vascular dysfunction.

Objective: To examine the relationship between physical activity and total cholesterol levels, both partially and simultaneously, with VO?Max in women aged over 45 years in Malang City.

Methods: A quantitative cross-sectional study following STROBE guidelines recruited 20 women aged over 45 via purposive sampling. Physical activity was measured using the IPAQ, total cholesterol via a digital capillary analyser, and VO?Max via the 6-Minute Walk Test. Data were analysed using descriptive statistics, Kolmogorov–Smirnov normality test, linearity tests, Pearson correlation, and multiple correlation.

Results: Physical activity was strongly and positively correlated with VO?Max (r = 0.785; p < 0.001). Total cholesterol was weakly and negatively correlated with VO?Max (r = ?0.287; p = 0.220), a non-significant association. Simultaneously, physical activity and total cholesterol showed a strong relationship with VO?Max (R = 0.790; R² = 0.624; p < 0.001), with physical activity contributing the dominant unique variance (54.2%) compared with total cholesterol (0.8%).

Conclusion: Physical activity is the primary determinant of VO?Max in women over 45, while total cholesterol shows a weak, non-significant negative association. Together, both variables explain 62.4% of VO?Max variance. Interventions should prioritise structured physical activity programmes integrated with lipid management.

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References

Almuraikhy, S., Sellami, M., Haddad, M., Anwardeen, N., Al-Mohannadi, M., & Elrayess, M. (2026). Age-Dependent Differences in Exercise Response Among Healthy Women: Impact on Inflammation, Lipids Profile and Glucose. Biomedicines, 14. https://doi.org/10.3390/biomedicines14030575

Badon, S., Gabriel, K., Karvonen-Gutierrez, C., Sternfeld, B., Gold, E., Waetjen, L., Lee, C., Avalos, L., Khoudary, S. El, & Hedderson, M. (2021). Dual trajectories of physical activity and blood lipids in midlife women: The Study of Women’s Health Across the Nation. Maturitas, 146, 49–56. https://doi.org/10.1016/j.maturitas.2021.02.002

Ballesta-García, I., Martínez-González-Moro, I., Ramos-Campo, D., & Carrasco-Poyatos, M. (2020). High-Intensity Interval Circuit Training Versus Moderate-Intensity Continuous Training on Cardiorespiratory Fitness in Middle-Aged and Older Women: A Randomized Controlled Trial. International Journal of Environmental Research and Public Health, 17. https://doi.org/10.3390/ijerph17051805

Baptista, L., Machado-Rodrigues, A., Da Silva, M., De França, E., & Martins, R. (2026). Sex Patterns of Statin Therapy and Multicomponent Exercise Training on Cardiorespiratory Fitness in Older Adults with Dyslipidemia: A 24-Month Cohort Study. Sports, 14. https://doi.org/10.3390/sports14060215

Bar, A., Berkowicz, P., Kurpi?ska, A., Mohaissen, T., Kara?, A., Kaczara, P., Suraj?Pra?mowska, J., Sternak, M., Marczyk, B., Malinowska, A., Kij, A., Jasztal, A., Czy?y?ska-Cicho?, I., Pieterman, E., Princen, H., Wi?niewski, J., & Ch?opicki, S. (2025). Effects of life-long hyperlipidaemia on age-dependent development of endothelial dysfunction in humanised dyslipidaemic mice. GeroScience, 47, 2673–2701. https://doi.org/10.1007/s11357-025-01578-w

Bernal, J., Veiga, A., Andrade, G., Rodrigues, M., & De Souza, H. (2025). Effects of physical exercise on the lipid profile of perimenopausal and postmenopausal women: a systematic review and meta-analysis. Brazilian Journal of Medical and Biological Research, 58. https://doi.org/10.1590/1414-431x2025e14194

Braga, P., Freitas, F., Bachi, A., Amirato, G., Baroni, R., Alves, M., Vieira, R., Vaisberg, M., Aldin, M., Kalil, R., Figueiredo, A., Damasceno, N., Tavoni, T., & Maranhão, R. (2023). Regular Practice of Physical Activity Improves Cholesterol Transfers to High-Density Lipoprotein (HDL) and Other HDL Metabolic Parameters in Older Adults. Nutrients, 15. https://doi.org/10.3390/nu15234871

Campos-Hernández, C., Romero-García, T., Frayde-Gómez, H., López-Campos, C. E., Navarro-Ibarra, M., Borbón-Román, J. C., Machado-Parra, J., Porras-Alvarado, V. E., & Oregel-Cortez, M. I. (2026). Effects of 12-Week Multicomponent Training Program on Body Composition, Metabolic Health, and Physical Performance in Middle-Aged and Older Women: Exploratory Role of Baseline Adiposity. Sports, 14. https://doi.org/10.3390/sports14050204

Carrick-Ranson, G., Howden, E., Brazile, T., Levine, B., & Reading, S. (2023). Effects of Aging and Endurance Exercise Training on Cardiorespiratory Fitness and Cardiac Structure and Function in Healthy Midlife and Older Women. Journal of Applied Physiology. https://doi.org/10.1152/japplphysiol.00798.2022

Da Silva Carvalho, G., Pasin, J. K., Valter, L., Kaminski, M., Spielmann, F. V. J., & Wender, M. (2025). Impact of aerobic exercise on cardiovascular and mental health in postmenopausal women: a systematic review and meta-analysis. Menopause, 32 8, 779–789. https://doi.org/10.1097/gme.0000000000002552

Dashti, A., Rahimi, N. M., & Azimkhani, A. (2025). Effects of Functional Training on Physical Function, Cardiorespiratory Fitness, and Quality of Life in Older Women: A 6-Week Randomized Controlled Trial. Biological Research For Nursing, 27, 570–580. https://doi.org/10.1177/10998004251337063

Figueira, B., Bravo, J., Raimundo, A., & Reis, A. (2025). Effects of a 26 week multicomponent exercise program on cardiovascular and lipid profiles in premenopausal and postmenopausal women. Scientific Reports, 15. https://doi.org/10.1038/s41598-025-05378-2

Harun, I., Riyadi, H., Briawan, D., & Khomsan, A. (2022). Effect of 12-Weeks Brisk Walking Exercise Duration on Blood Pressure and VO2max on Overweight and Obese Female Students in Indonesia. Nutrición Clínica y Dietética Hospitalaria. https://doi.org/10.12873/424harun

He, M.-J., Hu, S., Wang, J., Wang, J., G?man, M., Hariri, Z., & Tian, Y. (2023). Effect of resistance training on lipid profile in postmenopausal women: A systematic review and meta-analysis of randomized controlled trials. European Journal of Obstetrics, Gynecology, and Reproductive Biology, 288, 18–28. https://doi.org/10.1016/j.ejogrb.2023.06.023

Higashi, Y. (2023). Endothelial Function in Dyslipidemia: Roles of LDL-Cholesterol, HDL-Cholesterol and Triglycerides. Cells, 12. https://doi.org/10.3390/cells12091293

Huynh, E., Wiley, E., Noguchi, K., Fang, H., Beauchamp, M., MacDonald, M., & Tang, A. (2024). The effects of aerobic exercise on cardiometabolic health in postmenopausal females: A systematic review and meta-analysis of randomized controlled trials. Women’s Health, 20. https://doi.org/10.1177/17455057241290889

Kalanin, P. M., & Uher, I. (2026). Real-World Associations Between Physical Activity, LDL Cholesterol, and Functional Performance in Primary Care: A Cross-Sectional Study. Healthcare, 14. https://doi.org/10.3390/healthcare14111522

Karvinen, S., Jergenson, M., Hyvärinen, M., Aukee, P., Tammelin, T., Sipilä, S., Kovanen, V., Kujala, U., & Laakkonen, E. (2019). Menopausal Status and Physical Activity Are Independently Associated With Cardiovascular Risk Factors of Healthy Middle-Aged Women: Cross-Sectional and Longitudinal Evidence. Frontiers in Endocrinology, 10. https://doi.org/10.3389/fendo.2019.00589

Khalafi, M., Sakhaei, M., Maleki, A. H., Rosenkranz, S., Pourvaghar, M., Fang, Y., & Korivi, M. (2023). Influence of exercise type and duration on cardiorespiratory fitness and muscular strength in post-menopausal women: a systematic review and meta-analysis. Frontiers in Cardiovascular Medicine, 10. https://doi.org/10.3389/fcvm.2023.1190187

Niu, Y.-T., Radzimi?ski, ?., Qian, G.-P., Szwarc, A., & Ossowski, Z. (2025). Effect of a 12-week bungy pump training on cardiorespiratory fitness, metabolic syndrome factors and body composition in postmenopausal women. Scientific Reports, 15. https://doi.org/10.1038/s41598-025-25909-1

Nodeland, M., Klevjer, M., Sæther, J., Giskeødegård, G., Bathen, T., Wisløff, U., & Bye, A. (2022). Atherogenic lipidomics profile in healthy individuals with low cardiorespiratory fitness: The HUNT3 fitness study. Atherosclerosis, 343, 51–57. https://doi.org/10.1016/j.atherosclerosis.2022.01.001

Oh, D.-H., & Lee, J.-K. (2023). Effect of Different Intensities of Aerobic Exercise Combined with Resistance Exercise on Body Fat, Lipid Profiles, and Adipokines in Middle-Aged Women with Obesity. International Journal of Environmental Research and Public Health, 20. https://doi.org/10.3390/ijerph20053991

Park, H.-Y., Jung, W.-S., Kim, S.-W., Jung, K., & Lim, K.-Y. (2022). Comparison of Vascular Function, Cardiometabolic Parameters, Hemorheological Function, and Cardiorespiratory Fitness Between Middle-Aged Korean Women With and Without Obesity—A Pilot Study. Frontiers in Physiology, 13. https://doi.org/10.3389/fphys.2022.809029

Ramos-Campo, D., Andreu-Caravaca, L., Carrasco-Poyatos, M., Benito, P., & Rubio-Arias, J. (2021). Effects of Circuit Resistance Training on Body Composition, Strength, and Cardiorespiratory Fitness in Middle-Aged and Older Women: A Systematic Review and Meta-Analysis. Journal of Aging and Physical Activity, 1–14. https://doi.org/10.1123/japa.2021-0204

Ruiz-Rios, M., & Maldonado-Martín, S. (2022). Physical activity on cardiorespiratory fitness and cardiovascular risk in premenopausal and postmenopausal women: a systematic review of randomized controlled trials. Menopause, 29, 1222–1229. https://doi.org/10.1097/gme.0000000000002037

Shen, J., Peng, J., Luan, Y., Liu, X.-Y., Li, J., & Wu, J. (2026). Effects of exercise on metabolic risk, cardiovascular fitness, and body composition in elderly women of the past decade: a systematic review and meta-analysis. Journal of the International Society of Sports Nutrition, 23. https://doi.org/10.1080/15502783.2026.2675444

Souza, V. R. D. A., Vivan, L., Seffrin, A., Vallini, L., De Paula Domingos, F., De Lira, C., Vancini, R., Weiss, K., Rosemann, T., Knechtle, B., & Andrade, M. (2024). Impact of aging on maximal oxygen uptake in female runners and sedentary controls. Experimental Gerontology, 112476. https://doi.org/10.1016/j.exger.2024.112476

Van Lennep, J. R., Tokgözo?lu, L., Badimón, L., Dumanski, S., Gulati, M., Hess, C., Holven, K., Kavousi, M., Kay?kç?o?lu, M., Lutgens, E., Michos, E., Prescott, E., Stock, J., Tybjaerg-Hansen, A., Wermer, M., & Benn, M. (2023). Women, lipids, and atherosclerotic cardiovascular disease: a call to action from the European Atherosclerosis Society. European Heart Journal, 44, 4157–4173. https://doi.org/10.1093/eurheartj/ehad472

Wang, C., Yang, N., Zhang, Q., Li, Y., Zhang, L., Liu, Z., Zhao, R., Qi, J., Xiang, A., Fu, Y.-X., Xiong, Z., Wu, L., Sun, J., & Huang, D. (2025). Association between metabolic health indicators and cardiorespiratory fitness in urban young and middle-aged population: a retrospective cross-sectional study. Frontiers in Endocrinology, 16. https://doi.org/10.3389/fendo.2025.1489152

Woodward, A., Mason-Jones, A., Faires, M., Jones, V., & Beaumont, A. (2025). Effect of Physical Activity on Cardiorespiratory Fitness and Markers of Cardiovascular Disease Risk During Menopause: A Systematic Review and Meta-Analysis of Randomised-Controlled Trials. Journal of Science in Sport and Exercise, 8, 9–31. https://doi.org/10.1007/s42978-025-00343-x

Wu, X., Wilke, M., Batara, J., Proctor, S., Cree, M., Ghosh, M., Raggi, P., Windram, J., Becher, H., & Vine, D. (2025). Atherogenic ApoB-dyslipidemia, atherosclerotic cardiovascular disease, cardiac dysfunction and remodeling in high-risk young women with and without polycystic ovary syndrome: A pilot study. Frontiers in Endocrinology, 16. https://doi.org/10.3389/fendo.2025.1520922

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How to Cite

Pujianto, M. E. C., & Kinanti, R. G. (2026). Association of Physical Activity and Total Cholesterol with VO2Max in Middle-Aged Women: A Cross-Sectional Study. Health Frontiers: Multidisciplinary Journal for Health Professionals, 4(Suppl 1), S65–S76. https://doi.org/10.62255/mjhp.v4iSuppl 1.325