Menstrual Duration and Pad Replacement Frequency Predict Anemia Risk in Adolescent Girls: A Multivariate Cross-Sectional Analysis
DOI :
https://doi.org/10.62255/mjhp.v4iSuppl%201.335Abstract
Background: Anemia affects over 500 million adolescent girls globally, with menstruation representing a modifiable contributor to iron depletion. Despite the biological plausibility linking menstrual bleeding patterns to hemoglobin status, evidence integrating multiple menstrual characteristics within multivariate predictive frameworks remains scarce, particularly in community-based populations of low- and middle-income countries
Objective: To examine the independent associations between menstrual characteristics and anemia risk among adolescent girls, controlling for nutritional and sociodemographic confounders.
Methods: A community-based cross-sectional study was conducted among 75 adolescent girls in Ganjaran Village, Malang, Indonesia, following STROBE guidelines. Hemoglobin levels were measured via digital hemoglobinometry, and menstrual characteristics were assessed through structured questionnaires. Bivariate associations were evaluated using Spearman's correlation and independent t-tests, while multivariate logistic regression was applied to estimate adjusted odds ratios (aOR) for anemia (Hb < 12 g/dL).
Results: The prevalence of anemia was 57.3%. Menstrual duration >7 days (aOR = 6.42, 95% CI: 1.85–22.31, p = 0.003), sanitary pad replacement >5 times/day (aOR = 4.18, 95% CI: 1.32–13.26, p = 0.015), and irregular menstrual cycles (aOR = 2.94, 95% CI: 1.08–8.02, p = 0.035) were independent predictors of anemia. Low dietary iron intake and non-adherence to iron supplementation further amplified risk.
Conclusion: Menstrual bleeding patterns, particularly duration and frequency of pad replacement, independently predict anemia risk beyond nutritional factors. Integrating menstrual history into adolescent anemia screening programs may enable earlier identification of at-risk populations.
Downloads
Keywords
References
Amartha, T. A. S., Astuti, V. A., & Prastika, D. (2025). DETERMINANTS OF ANEMIA AMONG ADOLESCENT GIRLS. Journal of Applied Health Management and Technology. https://doi.org/10.31983/jahmt.v7i2.14050
Andriastuti, M., Ilmana, G., Nawangwulan, S. A., & Kosasih, K. (2019). Prevalence of anemia and iron profile among children and adolescent with low socio-economic status. International Journal of Pediatrics & Adolescent Medicine, 7, 88–92. https://doi.org/10.1016/j.ijpam.2019.11.001
Astikasari, N. D., Wahyuni, C., Ripursari, T., & Mandasari, D. (2025). Factor Analysis of Menstrual Duration, Level of Knowledge, and Compliance with the Consumption of Blood Supplement Tablets on the Prevalence of Anemia in Adolescents with Prostate. Journal Of Nursing Practice. https://doi.org/10.30994/jnp.v8i3.837
Bali, S., Sembiah, S., Jain, A., Alok, Y., Burman, J., & Parida, D. (2021). Is There Any Relationship between Poor Menstrual Hygiene Management and Anemia? – A Quantitative Study Among Adolescent Girls of the Urban Slum of Madhya Pradesh. Indian Journal of Community Medicine?: Official Publication of Indian Association of Preventive & Social Medicine, 46, 550–553. https://doi.org/10.4103/ijcm.ijcm_73_21
Berhe, K., Gebrearegay, F., Gebreegziabher, H., Weldegerima, L., Kahsay, A., Hadush, H., Gebremariam, B., Fseha, B., Gebremariam, G., Etsay, N., & Hailu, M. (2022). Magnitude and associated factors of anemia among adolescent girls in Ethiopia: a systematic review and meta-analysis. Archives of Public Health, 80. https://doi.org/10.1186/s13690-022-00942-y
Chyntaka, M., Irbah, L., & Putri, N. (2026). HUBUNGAN POLA MENSTRUASI DENGAN KEJADIAN ANEMIA PADA REMAJA PUTRI. Optimal Midwife & Nursing Journal. https://doi.org/10.67717/740734
Duggirala, S., Bose, S., & Karuturi, R. (2026). Burden and Associated Factors of Anaemia Among Rural School-Going Adolescent Girls in North Coastal Andhra Pradesh, India: A Cross-Sectional Study. Cureus, 18. https://doi.org/10.7759/cureus.111649
Ekasanti, I., Adi, A., Yono, M., G., N., & Isfandiari, M. (2020). Determinants of Anemia among Early Adolescent Girls in Kendari City. Amerta Nutrition. https://doi.org/10.20473/amnt.v4i4.2020.271-279
Endale, F., Woldeyohannes, D., Belayneh, F., Tamene, A., Habte, A., Gizachew, A., Sulamo, D., Kebede, Y., Yohannes, J., Mekonen, T., & Akiso, D. (2022). Menstrual abnormality, maternal illiteracy, and household factors as main predictors of anemia among adolescent girls in Ethiopia: Systematic review and meta-analysis. Women’s Health, 18. https://doi.org/10.1177/17455057221129398
Fitriyanti, N., & Achadi, A. (2024). Factors Associated to the Incidence of Anemia Among Adolescent Girls in Muaragembong District Bekasi Regency. Journal of Public Health for Tropical and Coastal Region. https://doi.org/10.14710/jphtcr.v7i3.24877
Fuadah, F., & Saragih, B. (2024). Analysis of Factors in Reducing the Incidence of Anemia in Adolescent Girls at Ummi Kulsum Banjaran SMP Bandung District. International Journal of Current Science Research and Review. https://doi.org/10.47191/ijcsrr/v7-i6-61
Ghimire, M., Bhandari, S., & Rajbanshi, M. (2024). Prevalence of anemia and its associated factors among school-going adolescent girls in schools of Dhankuta municipality, Nepal. PLOS Global Public Health, 4. https://doi.org/10.1371/journal.pgph.0003684
Ginting, A., Andrafikar, Puspita, R., Ariani, R., & Rahayu, E. S. (2026). Factors Associated with Anemia in Adolescent Girls: An Analysis of the Role of Diet, Physical Activity and Adherence to Iron Supplements at Sehati Vocational School, Karawang. Miracle Get Journal. https://doi.org/10.69855/mgj.v3i3.773
Karinda, M., Lellyawaty, L., & Vaira, R. (2025). Analysis of Menstrual Periods, Menstrual Cycle, Menstrual Pain and Body Mass Index with the Incidence of Anemia in Adolescent Girls. Biomedical Journal of Indonesia. https://doi.org/10.32539/bji.v11i1.222
Kebede, W., Hailu, A. G., Hegena, T., & Agde, Z. (2025). Prevalence of anemia and its associated factors among rural adolescent girls in West Badewacho district, central Ethiopia: a community based cross-sectional study. Frontiers in Public Health, 13. https://doi.org/10.3389/fpubh.2025.1567419
Khoirunnisa, N., Nurhartanto, A., & Astuti, D. (2025). The Relationship Between Iron-Rich Food Consumption and Iron Supplement Adherence with the Incidence of Anemia among Adolescent. Genius Journal. https://doi.org/10.56359/gj.v7i1.785
Kusumawardani, D. A., Ningsih, A. H., & Adi, D. I. (2026). Dietary patterns and menstrual cycle: incidence of anemia in female adolescents in Bondowoso Regency. Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition). https://doi.org/10.14710/jgi.14.2.208-218
Mengistu, G., Azage, M., & Gutema, H. (2019). Iron Deficiency Anemia among In-School Adolescent Girls in Rural Area of Bahir Dar City Administration, North West Ethiopia. Anemia, 2019. https://doi.org/10.1155/2019/1097547
Nasrul, N., Hafid, F., Taqwin, T., Hikmah, Risma, Nuswantari, A., & Sariman, S. (2025). Iron Supplement Consumption, Dietary Intake, Nutritional Status, and Anemia Among Adolescent Girls in Banawa, Donggala Regency. Journal of Health and Nutrition Research. https://doi.org/10.56303/jhnresearch.v4i2.549
Nuzullifa, Y., & Fadjri, T. (2025). Correlation Between Menstrual Cycle with Anemia Among Female Adolescents at SMA Negeri 1 Meukek, South Aceh. JAND: Journal of Applied Nutrition and Dietetic. https://doi.org/10.30867/jand.v5i1.223
Puspitasari, E., Zaman, C., Harokan, A., & Suryani, D. (2025). Analysis of factors affecting the incidence of anemia in adolescent girls at Nawangsasi Health Center: A cross-sectional study. Lentera Perawat. https://doi.org/10.52235/lp.v6i3.485
Putri, F., Suyanto, S., Restila, R., Laksono, A., & Sundjaya, T. (2025). Exploring the role of nutritional status and anthropometric factors in anemia among adolescent girls in Pekanbaru, Indonesia. SAGE Open Medicine, 13. https://doi.org/10.1177/20503121251355406
Putri, T., Nuraeni, N., & Nurlina, F. (2024). MENSTRUAL CYCLE AND THE INCIDENT OF ANEMIA IN TEENAGE WOMEN. Journal of Maternity Care and Reproductive Health. https://doi.org/10.36780/jmcrh.v6i4.12268
Rahfiludin, M., Arso, S. P., Joko, T., Asna, A. F., Murwani, R., & Hidayanti, L. (2021). Plant-based Diet and Iron Deficiency Anemia in Sundanese Adolescent Girls at Islamic Boarding Schools in Indonesia. Journal of Nutrition and Metabolism, 2021. https://doi.org/10.1155/2021/6469883
Ramadhani, K., Penak, R. F., Muthi’ah, T. S., & Ayuningtyas, C. (2026). Association between Iron and Folic Acid Supplementation Adherence and Anemia among Adolescent Girls in Kulon Progo, Indonesia. Al GIZZAI: Public Health Nutrition Journal. https://doi.org/10.24252/algizzai.v6i1.63152
Sari, C. C., & Ritunga, I. (2025). Analisis Hubungan Pola Menstruasi dan Pengetahuan Remaja Terhadap Kejadian Anemia pada Remaja Putri di SMA Darut Taqwa Purwosari Tahun 2024. Prominentia Medical Journal. https://doi.org/10.37715/pmj.v6i2.5710
Sari, P., Herawati, D., Dhamayanti, M., & Hilmanto, D. (2022). Anemia among Adolescent Girls in West Java, Indonesia: Related Factors and Consequences on the Quality of Life. Nutrients, 14. https://doi.org/10.3390/nu14183777
Sari, P., Judistiani, R., Herawati, D., Dhamayanti, M., & Hilmanto, D. (2022). Iron Deficiency Anemia and Associated Factors Among Adolescent Girls and Women in a Rural Area of Jatinangor, Indonesia. International Journal of Women’s Health, 14, 1137–1147. https://doi.org/10.2147/ijwh.s376023
Sigit, F., Ilmi, F. B., Desfiandi, P., Saputri, D., Fajarini, N., Susianti, A., Lestari, L., & Faras, A. (2023). Factors influencing the prevalence of anaemia in female adolescents: A population-based study of rural setting in Karanganyar, Indonesia. Clinical Epidemiology and Global Health. https://doi.org/10.1016/j.cegh.2023.101500
Silvia, A., Kartini, A., & Nugraheni, S. (2019). HUBUNGAN ASUPAN ZAT GIZI (PROTEIN, ZAT BESI, VITAMIN C) DAN POLA (SIKLUS, LAMA) MENSTRUASI DENGAN KADAR HEMOGLOBIN (Studi pada Remaja Putri di SMK Negeri 10 Semarang). 7, 504–516.
Söderman, L., Stubbendorff, A., Ladfors, L., Bolmsjö, B. B., Nymberg, P., & Wolff, M. (2025). Exploring the effect of menstrual loss and dietary habits on iron deficiency in teenagers: A cross-sectional study. PLOS One, 20. https://doi.org/10.1371/journal.pone.0336688
Suhariyati, S., Rahmawati, A., & Realita, F. (2020). Hubungan antara Pola Menstruasi dengan Kejadian Anemia pada Mahasiswi Prodi Sarjana Kebidanan Unissula Semarang. Jurnal Akademika Baiturrahim Jambi. https://doi.org/10.36565/jab.v9i2.214
Tando, Y., Titaley, C., Tahitu, R., Asmin, E., & Sara, L. (2024). Anemia and adherence to weekly iron-folic acid supplementation among adolescent girls in a stunting locus of Ambon City, Indonesia. Journal of Health Research. https://doi.org/10.56808/2586-940x.1103
Taufiqurrahman, T., Hafid, F., Mujayanto, M., Nuswantari, A., Ratno, R., Rahayuningtyas, R., & Prasetyawan, A. (2025). Iron Supplement Adherence, Dietary Intake, and Anemia Risk among Adolescent Girls in Madiun Regency, Indonesia. Journal of Health and Nutrition Research. https://doi.org/10.56303/jhnresearch.v4i3.723
Utama, F., Rahmiwati, A., & Arinda, D. F. (2020). Prevalence of Anaemia and its Risk Factors Among Adolescent Girls. Proceedings of the 2nd Sriwijaya International Conference of Public Health (SICPH 2019). https://doi.org/10.2991/ahsr.k.200612.066
Wahyurin, I., & Rahmah, H. A. (2021). Amount of Menstrual Blood and Nutrient Intake with Hemoglobin Level. Jurnal Kesehatan Masyarakat. https://doi.org/10.15294/kemas.v17i1.24540
Wati, E., Sistiarani, C., & Rahardjo, S. (2023). Diet behavior and consumption of iron inhibitors: Incidence anemia in adolescent girls. Journal of Public Health in Africa, 14. https://doi.org/10.4081/jphia.2023.2593
Wirth, J., Yarparvar, A., Galetti, V., El-Mallah, C., Boutros, M., Najjar, J., Mokdad, M. El, Kobayter, D., Petry, N., Daou, M., Wakim, C., Asfahani, F., Abiad, F., & Obeid, O. (2025). Silent losses: predictors of anaemia and micronutrient deficiencies and their associations with menstrual bleeding in Lebanon – findings from a national cross-sectional study. BMJ Global Health, 10. https://doi.org/10.1136/bmjgh-2025-020251
Yadav, P., Kumar, A., & Kansal, S. (2023). Determinants of anaemia among adolescent girls in rural Varanasi, Uttar Pradesh. International Journal of Research in Medical Sciences. https://doi.org/10.18203/2320-6012.ijrms20232159
Yalestyarini, E. A., Fibrinia, A. A., Saputro, H., & Fazrin, I. (2026). Association between Iron Tablet Adherence and Anemia among Adolescent Girls. Journal Of Nursing Practice. https://doi.org/10.30994/jnp.v9i3.1151
Downloads
How to Cite
Issue
Section
License
Copyright (c) 2026 Niken Parahita Kusumaningtyas, Taurisma Aulia Nanda Wibisono, Syafira Assary Budiman, Tisnalia Merdya Andyastanti

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License .
You are free to:
• Share — copy and redistribute the material in any medium or format.
• Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Under the following terms:
• Attribution — You must give appropriate credit to the original author(s) and Health Frontiers, provide a link to the license, and indicate if changes were made.
• ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.




